Immunohistochemical identification of GM-CSF in testis of rats with oligozoospermia with the intervention of Cuscuta chinensis flavonoids / Identificación inmunohistoquímica de GM-CSF en testículos de ratas con oligozoospermia con la intervención de flavonoides de Cuscuta chinensis

Oligozoospermia is a common infertility disease, and the incidence rate is increasing year by year. Cuscuta chinensis is a commonly used medicine for the treatment of oligozoospermia in Chinese medicine. Flavonoids are its main component. GM-CSF is a multifunctional cytokine that plays an important role in the inflammatory response. In this paper, we performed HE staining and immunohistochemical staining on the testis of rats with oligozoospermia. We intend to study the expression changes of GM-CSF in rats with oligospermia and the effect of flavonoids on the expression of GM-CSF in testis of rats with oligozoospermia.

La oligozoospermia es una enfermedad común de infertilidad, con una tasa de incidencia que aumenta año tras año. Cuscuta chinensis es un medicamento de uso común para el tratamiento de la oligozoospermia en la medicina china. Los flavonoides son su componente principal. GM-CSF es una citocina multifuncional que tiene un rol importante en la respuesta inflamatoria. En este trabajo, realizamos tinción con hematoxilina y eosina y tinción inmunohistoquímica en testículos de ratas con oligozoospermia. TNuestro objetivo fue estudiar los cambios de expresión de GM-CSF en ratas con oligozoospermia y el efecto de los flavonoides en la expresión de GM-CSF en testículos de ratas con oligozoospermia.

The Effect by of Inspiring High Concentration Oxygen on the Reactive Oxygen Species in the Rats Brain Homogenates / 医学研究杂志

Objective To evaluate the security of inspiring high concentration oxygen by detecting the reactive oxygen species of the rats brain homogenates after inspiring high concentration oxygen for a certain time.Methods Sixty wister rats weighting 150~200g were randomly devided into five groups(the group of 4h、8h、12h、16h and the control group,12/group).The experimental rats were exposed to 85%~100% oxygen in a self-made chamber and the control animals were kept in normal room air.The Concentration of the MDA,SOD,GSH,NO,NOS,CAT and T-AOC of the brain homogenates was detected.Results Compared with the control group,the level of MDA,NO and NOS in the brain homogenates grew up markedly(P